Training Programs –

High Performance Liquid Chromatography (HPLC)

Job Description

Designation : HPLC Trainee
Section : Training
Qualification : Ph. D./ M. Tech./ M. Sc./ B. Tech./ B. Sc.
Experience : Fresher
Employment Type : Short Term / Long Term
Location : Gurugram, Haryana

Description

High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for separating, identifying, and quantifying components in a mixture. Utilizing high pressure to force a liquid mobile phase through a stationary phase, HPLC effectively separates compounds based on their interactions with both phases. This method is highly versatile, allowing for the analysis of a wide range of substances, including pharmaceuticals, food additives, and environmental samples. HPLC is renowned for its speed, precision, and sensitivity, making it an essential tool in laboratories for quality control, research, and development across various industries.
FARE Labs conducts a specialized training on High-Performance Liquid Chromatography (HPLC) tailored for various sample matrices following national/ international standard methodology. This training is aimed at laboratory professionals, researchers, and students interested in analyzing food additives such as antioxidants, synthetic colors, preservatives, artificial sweeteners etc.

Objectives
1. Provide a solid understanding of HPLC principles and applications in food safety.
2. Equip trainees with practical skills for operating HPLC systems.
3. Discuss the analysis of specific food additives using HPLC.
4. Familiarize attendees with data analysis and interpretation techniques.

What is to learn?

1. Introduction to HPLC
Overview of chromatography principles and HPLC components (pumps, injectors, columns, detectors)

2. Instrumentation

Pumps: Isocratic vs. gradient systems
Injectors: Manual vs. automatic
Columns: Selection criteria for food analysis
Detectors: UV-Vis, fluorescence, ELSD, and mass spectrometry

3. HPLC Method Development for Food Additives
Identification and quantification of various target analytes such as:

Antioxidants: TBHQ, BHT, BHA etc.
Synthetic Colours: eight permitted synthetic colours, banned colours
Preservatives: benzoates, sorbates etc.
Artificial Sweeteners: Aspartame, saccharin etc.

Practical Applications

1. Hands-On Training

Instrument setup and calibration for food analysis
Preparation and analysis of more than 500 samples of various matrices
Running HPLC methods for food additives

2. Data Analysis

Understanding chromatograms and quantification methods
Software tools for data processing and reporting

3. Troubleshooting Common Issues

Resolving baseline noise, peak tailing, and fronting
Preventive maintenance tips for HPLC systems

Responsibility

Adherence to Safety Protocols: Strictly follow all laboratory safety guidelines and protocols to prevent accidents and ensure the well-being of all participants.
Active Participation: Engage actively in all training sessions, discussions, and practical exercises. Complete all assigned tasks and projects on time.
Respect for Equipment and Facilities: Handle all laboratory equipment and materials with care. Report any damages or malfunctions to the instructor immediately.
Collaboration and Teamwork: Work collaboratively with fellow participants, sharing knowledge and supporting each other’s learning.
Integrity and Accuracy: Maintain a high standard of integrity in all testing procedures and documentation. Ensure that all results are accurate and reliable.

Requirements - Skills, Knowledge and Abilities

Commitment to the Program: Participants must demonstrate a commitment to the training program by attending all scheduled sessions, completing all assignments, and actively participating in practical exercises. A signed commitment agreement may be required before the start of the program.
Health and Safety Compliance: Participants must comply with health and safety regulations, including wearing appropriate personal protective equipment (PPE) and adhering to all safety protocols. A medical clearance or proof of immunizations may be required for certain training programs.