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Training Programs –

High Performance Liquid Chromatography (HPLC)

Job Description

  • Designation : HPLC Trainee
  • Section : Training
  • Qualification : Ph. D./ M. Tech./ M. Sc./ B. Tech./ B. Sc.
  • Experience : Fresher
  • Employment Type : Short Term / Long Term
  • Location : Gurugram, Haryana

High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for separating, identifying, and quantifying components in a mixture. Utilizing high pressure to force a liquid mobile phase through a stationary phase, HPLC effectively separates compounds based on their interactions with both phases. This method is highly versatile, allowing for the analysis of a wide range of substances, including pharmaceuticals, food additives, and environmental samples. HPLC is renowned for its speed, precision, and sensitivity, making it an essential tool in laboratories for quality control, research, and development across various industries.
FARE Labs conducts a specialized training on High-Performance Liquid Chromatography (HPLC) tailored for various sample matrices following national/ international standard methodology. This training is aimed at laboratory professionals, researchers, and students interested in analyzing food additives such as antioxidants, synthetic colors, preservatives, artificial sweeteners etc.

Objectives
1. Provide a solid understanding of HPLC principles and applications in food safety.
2. Equip trainees with practical skills for operating HPLC systems.
3. Discuss the analysis of specific food additives using HPLC.
4. Familiarize attendees with data analysis and interpretation techniques.

1. Introduction to HPLC
Overview of chromatography principles and HPLC components (pumps, injectors, columns, detectors)

2. Instrumentation

  • Pumps: Isocratic vs. gradient systems
  • Injectors: Manual vs. automatic
  • Columns: Selection criteria for food analysis
  • Detectors: UV-Vis, fluorescence, ELSD, and mass spectrometry

 

3. HPLC Method Development for Food Additives
Identification and quantification of various target analytes such as:

  • Antioxidants: TBHQ, BHT, BHA etc.
  • Synthetic Colours: eight permitted synthetic colours, banned colours
  • Preservatives: benzoates, sorbates etc.
  • Artificial Sweeteners: Aspartame, saccharin etc.

1. Hands-On Training

  • Instrument setup and calibration for food analysis
  • Preparation and analysis of more than 500 samples of various matrices
  • Running HPLC methods for food additives

 

2. Data Analysis

  • Understanding chromatograms and quantification methods
  • Software tools for data processing and reporting

 

3. Troubleshooting Common Issues

  • Resolving baseline noise, peak tailing, and fronting
  • Preventive maintenance tips for HPLC systems

Responsibility

  1. Adherence to Safety Protocols: Strictly follow all laboratory safety guidelines and protocols to prevent accidents and ensure the well-being of all participants.
  2. Active Participation: Engage actively in all training sessions, discussions, and practical exercises. Complete all assigned tasks and projects on time.
  3. Respect for Equipment and Facilities: Handle all laboratory equipment and materials with care. Report any damages or malfunctions to the instructor immediately.
  4. Collaboration and Teamwork: Work collaboratively with fellow participants, sharing knowledge and supporting each other’s learning.
  5. Integrity and Accuracy: Maintain a high standard of integrity in all testing procedures and documentation. Ensure that all results are accurate and reliable.

Requirements - Skills, Knowledge and Abilities

  1. Commitment to the Program: Participants must demonstrate a commitment to the training program by attending all scheduled sessions, completing all assignments, and actively participating in practical exercises. A signed commitment agreement may be required before the start of the program.
  2. Health and Safety Compliance: Participants must comply with health and safety regulations, including wearing appropriate personal protective equipment (PPE) and adhering to all safety protocols. A medical clearance or proof of immunizations may be required for certain training programs.
Get in Touch
Email at trainings@farelabs.com
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