Practical Applications

1. Hands-On Training Sample preparation for RTPCR and ELISA from different food matrices DNA isolation using various methods and quantification. Preparation and optimisation of RTPCR reactions. Setting up RTPCR reactions for detection of GMO elements such as 35S promoter and NoS terminator. Running RTPCR experiments and analysing real-time fluorescence data. Performing ELISA assays for antigen-antibody interactions. Step-by-step guidance on data acquisition and interpretation. 2. Data Analysis and Interpretation Understanding amplification curves and threshold cycles (Ct values) to assess the presence or absence of specific GMO traits such as 35S promoter and NoS terminator. Detection of GMO content using standard curves and reference genes. ELISA result quantification using standard curves. Software tools for data processing and statistical analysis. 3. Troubleshooting Common Issues Identifying and resolving common problems (e.g., concentration, contamination, false positives/negatives). Strategies for improving assay sensitivity and specificity. Preventive maintenance tips for equipment longevity.

Adherence to Safety Protocols : Strictly follow all laboratory safety guidelines and protocols to prevent accidents and ensure the well-being of all participants. Active Participation: Engage actively in all training sessions, discussions, and practical exercises. Complete all assigned tasks and projects on time. Respect for Equipment and Facilities: Handle all laboratory equipment and materials with care. Report any damages or malfunctions to the instructor immediately. Collaboration and Teamwork: Work collaboratively with fellow participants, sharing knowledge and supporting each other’s learning. Integrity and Accuracy: Maintain a high standard of integrity in all testing procedures and documentation. Ensure that all results are accurate and reliable.

Requirements - Skills, Knowledge, and Abilities

Commitment to the Program: Participants must demonstrate a commitment to the training program by attending all scheduled sessions, completing all assignments, and actively participating in practical exercises. A signed commitment agreement may be required before the start of the program. Health and Safety Compliance: Participants must comply with health and safety regulations, including wearing appropriate personal protective equipment (PPE) and adhering to all safety protocols. A medical clearance or proof of immunizations may be required for certain training programs. Designation: Biotechnology Research Trainee Section: Training Qualification: Ph.D./M.Tech./M.Sc./B.Tech./B. Sc. Fees : As Per Industry Standards Experience: Freshers / Professionals Employment Type: Short Term / Long Term Location: Gurugram, Haryana Note: FARE Labs also offers Summer Training Program for Biotechnology Limited Spots Available! Don’t Miss This Opportunity to Advance Your biotechnological Skills! For any further inquiries or additional information, please reach out. We look forward to supporting your journey in Biotechnology! FARE Labs – Empowering the Future of Biotechnology

Biotechnology Training at FARE Labs Pvt Ltd

Real Time-Polymerase Chain Reaction (RTPCR) and Enzyme-Linked Immunosorbent Assay (ELISA) are two powerful analytical techniques widely used in biotechnology for detecting nucleic acids and proteins, respectively. RT-PCR enables the amplification and quantification of DNA sequences, making it essential for genetic and diagnostic research. RT-PCR in food testing is particularly valuable for the detection of RNA viruses, bacterial pathogens (markers), spoilage organisms, and GMOs. It is also used for quantitative analysis of pathogens and microbial toxins. ELISA, on the other hand, is a highly sensitive immunological method used for detecting specific antigens or antibodies, playing a crucial role in disease diagnostics, food safety, and pharmaceutical research. ELISA is primarily used for detecting pathogens (bacteria, viruses), allergens, veterinary drug residues, and food adulterants. It is also used in food quality control and rapid screening.

FARE Labs having a state-of-the art accredited (ISO/IEC 17025:2017) Biotechnology Lab performing molecular analysis and GMO Testing, offers advanced Industrial Biotechnology Training Course covering Real Time Polymerase Chain Reaction (RTPCR) and Enzyme Linked Immunosorbent Assay (ELISA). This biotechnology training program aims to equip participants with essential knowledge and practical skills in RTPCR and ELISA techniques. It may be worth mentioning that the training course offered by FARE Labs is one of the best Biotechnology Training & Certification Program/Course on RTPCR and ELISA in Gurugram.

Objectives

Provide industrial biotechnology course
Training on RTPCR will provide a comprehensive understanding of RTPCR technique.
Provide Biotechnology ELISA testing procedure and interpretation training
Equip participants with practical skills for conducting RTPCR and ELISA assays.
Discuss method development and sample preparation techniques.
Familiarize attendees with data analysis, interpretation, and troubleshooting.

1. Introduction to RT-PCR and ELISA

Overview of molecular diagnostics and immunoassay techniques.
Principles of RTPCR and ELISA and their applications in biotechnology.
Comparison of RTPCR and ELISA with other diagnostic techniques.

2. Instrumentation and Setup

Key components of RTPCR: thermal cyclers, primers, and probes.
Overview of ELISA plate readers and reagents.
Sample preparation techniques for accurate results.

3. Method Development

Designing primers and probes for RTPCR.
Selection of antibodies and substrates for ELISA.
Optimization of reaction conditions for sensitivity and specificity.
Validation and calibration of assays.

4. Applications of RTPCR

Training will be provided on selected parameters from the list below:

Bacterial Food Pathogen Detection: Identifies pathogens like Salmonella, Listeria, E. coli O157:H7, Staphylococcus aureus, Clostridium botulinum by targeting DNA for rapid screening of food samples including meat, dairy, and processed products.
Genetically Modified Organism (GMO) Detection: Detects specific DNA sequences in genetically modified crops such as cereals, soy, cotton, herbs, spices, and animal food/feed.
Water Parasites Detection: Cryptosporidium and Giardia in drinking water samples.
Detection of Adulterants (Meat Authenticity Test): In meat and meat products.
Detection of Pork: Detects pork in meat and meat products to verify HALAL certification.
Vegan Claim testing: Confirms whether products are truly vegan by identifying specific genetic markers.
Detection of alleles and cow and buffalo milk: Milk & Dairy Products

5. Applications of ELISA:

Allergen Detection: Identifies allergens (peanuts, gluten, milk, eggs, tree nuts, fish, mustard, coconut, sesame seeds, sulphites) in food products such as dairy, fruits, vegetables, cereals, meat, bakery items, spices, herbs, and beverages.
Detection of enzymes: Lipase, amylase (animal food, oats) , protease (milk) .
Fish Toxins Detection: Tests for fish toxins like paralytic shellfish poison (PSP), Amensic shellfish poison (ASP), Diarrhetic shellfish poison (DSP), Brevetoxin (BTX) in seafood, including shrimp and prawns.
Food Quality Control & Traceability: Ensures food authenticity by detecting specific proteins or contaminants.
Enzyme Assays in Health Supplements: Applied in nutraceuticals, special dietary foods, and functional foods for enzyme activity testing.

Training & Certification Course in Biotechnology

Objectives

What is to Learn?

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