Training Programs –

Gas Chromatography Mass Spectrometry (GCMS)

Job Description

Description

Gas Chromatography-Mass Spectrometry (GC-MS) is a powerful analytical technique that combines the separation capabilities of gas chromatography with the identification and quantification strengths of mass spectrometry. In GC-MS, volatile compounds are vaporized and carried by an inert gas through a column where they are separated based on their chemical properties. The separated compounds then enter the mass spectrometer, which analyses their mass-to-charge ratios, providing detailed information about their structure. This method is widely used in fields such as environmental analysis, food safety, and forensic science due to its sensitivity, specificity, and ability to analyse complex mixtures.

FARE Labs provides advanced training on Gas Chromatography-Mass Spectrometry (GC-MS) on various sample matrices. GC-MS is a powerful analytical technique combining gas chromatography's ability to separate volatile compounds with mass spectrometry’s capability to identify and quantify those compounds.

Objectives
1. Provide a comprehensive understanding of GC-MS principles using national/ international standard methods.
2. Equip participants with practical skills for operating GC-MS systems.
3. Familiarize attendees with data analysis and interpretation techniques.

What is to learn?

1. Introduction to GC-MS

  • Overview of chromatography principles and HPLC components (pumps, injectors, columns, detectors).
  • Key components of a GC-MS system (injector, column, detector, mass analyzer).

 

2. Instrumentation

  • Gas Chromatography: Types of columns and their selection.
  • Mass Spectrometry: Ionization methods and mass analyzers (quadrupole, TOF).
  • Sample Introduction: Techniques for solid, liquid, and gas samples.

 

3. GC-MS Method Development
Identification of various target analytes such as:

  • Pesticides residual analysis of food.
  • Dioxins/Furans/PCBs in food and environment samples.
  • Aldrin, ethion, chlorpyriphos, phorate etc.

Practical Applications

1. Hands-On Training

  • Instrument setup and calibration.
  • Preparation and analysis of more than 500 samples of various matrices.
  • Running GC-MS methods.

 

2. Data Analysis

  • Understanding mass spectra and chromatograms.
  • Quantification methods and calibration curves.
  • Software tools for data processing and reporting.

 

3. Troubleshooting Common Issues

  • Resolving baseline noise and ion suppression.
  • Addressing peak resolution and sensitivity issues.
  • Preventive maintenance tips for GC-MS systems.

Responsibility

  1. Adherence to Safety Protocols: Strictly follow all laboratory safety guidelines and protocols to prevent accidents and ensure the well-being of all participants.
  2. Active Participation: Engage actively in all training sessions, discussions, and practical exercises. Complete all assigned tasks and projects on time.
  3. Respect for Equipment and Facilities: Handle all laboratory equipment and materials with care. Report any damages or malfunctions to the instructor immediately.
  4. Collaboration and Teamwork: Work collaboratively with fellow participants, sharing knowledge and supporting each other’s learning.
  5. Integrity and Accuracy: Maintain a high standard of integrity in all testing procedures and documentation. Ensure that all results are accurate and reliable.

Requirements - Skills, Knowledge and Abilities

  1. Commitment to the Program: Participants must demonstrate a commitment to the training program by attending all scheduled sessions, completing all assignments, and actively participating in practical exercises. A signed commitment agreement may be required before the start of the program.
  2. Health and Safety Compliance: Participants must comply with health and safety regulations, including wearing appropriate personal protective equipment (PPE) and adhering to all safety protocols. A medical clearance or proof of immunizations may be required for certain training programs.
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