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LCMS Training

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Liquid Chromatography-Mass Spectrometry (LC-MS) is a powerful analytical technique that combines the separation capabilities of liquid chromatography with the identification and quantification strengths of mass spectrometry. In LC-MS, complex mixtures are separated into individual components using liquid chromatography, after which mass spectrometry analyzes the separated compounds based on their mass-to-charge ratios. This method is highly sensitive and versatile, making it ideal for a wide range of applications, including pharmaceuticals, environmental monitoring, and food safety. LC-MS enables the detection of trace levels of compounds up to ppb levels, providing valuable insights into complex matrices in various scientific fields.

FARE Labs provides advanced training on Liquid Chromatography-Mass Spectrometry (LC-MS). This powerful analytical technique combines the separation capabilities of liquid chromatography with the identification and quantification strengths of mass spectrometry. LC-MS is widely used in various applications, including pharmaceuticals, environmental analysis, and food safety. This workshop aims to equip participants with essential knowledge and practical skills in LC-MS techniques.

Objectives

  1. Provide a thorough understanding of LC-MS principles and instrumentation.
  2. Equip participants with practical skills for operating and maintaining LC-MS systems.
  3. Discuss method development and sample preparation techniques.
  4. Familiarize attendees with data analysis, interpretation, and troubleshooting.

What is to learn?

1. Introduction to LC-MS

  • Overview of liquid chromatography and mass spectrometry
  • Principles of LC-MS and its applications in various fields
  • Key components of an LC-MS system (pump, injector, column, mass spectrometer)

2. Instrumentation and Setup

  • Detailed discussion of LC components: column selection, mobile phases, and detectorsTypes of mass spectrometers used with LC: quadrupole, time-of-flight (TOF)
  • Sample introduction systems

3. Method Development

  • Selecting appropriate analytical methods for different applications
  • Calibration techniques: standard curves and internal standards
  • Optimizing parameters: flow rates, column temperature, and ionization conditions

4. Applications of LC-MS/MS

  • Pesticide residues: Organochlorine, Organophosphate, Pyrethroids, Carbamates, Polar Pesticides
  • Antibiotics: Betalactum, aminoglycosides, macrolides, tetracyclines
  • Microtoxins: Aflatoxins, Deoxynivalenol (DON), Ochratoxin
  • Vitamins: Vitamin D2, Vitamin D3, Vitamin B7, Vitamin B9 and Vitamin B12
  • Hormones and steroids
  • Acrylamide
  • Melamine
  • Nitrosamines

1. Hands-On Training

  • Instrument setup and calibration for sample analysis
  • Preparation and analysis of more than 500 samples for various matrices
  • Running LC-MS methods: step-by-step guidance

2. Data Analysis and Interpretation

  • Understanding mass spectra and chromatograms
  • Quantification methods and calibration curve creation
  • Software tools for data processing and reporting

3. Troubleshooting Common Issues

  • Identifying and resolving common problems (e.g., baseline noise, peak tailing)
  • Strategies for maintaining instrument performance
  • Preventive maintenance tips
  1. Adherence to Safety Protocols: Strictly follow all laboratory safety guidelines and protocols to prevent accidents and ensure the well-being of all participants.
  2. Active Participation: Engage actively in all training sessions, discussions, and practical exercises. Complete all assigned tasks and projects on time.
  3. Respect for Equipment and Facilities: Handle all laboratory equipment and materials with care. Report any damages or malfunctions to the instructor immediately.
  4. Collaboration and Teamwork: Work collaboratively with fellow participants, sharing knowledge and supporting each other’s learning.
  5. Integrity and Accuracy: Maintain a high standard of integrity in all testing procedures and documentation. Ensure that all results are accurate and reliable.

Commitment to the Program: Participants must demonstrate a commitment to the training program by attending all scheduled sessions, completing all assignments, and actively participating in practical exercises. A signed commitment agreement may be required before the start of the program.

Health and Safety Compliance: Participants must comply with health and safety regulations, including wearing appropriate personal protective equipment (PPE) and adhering to all safety protocols. A medical clearance or proof of immunizations may be required for certain training programs.

Designation: LCMS Trainee
Section: Training
Qualification: Ph. D./ M. Tech./ M. Sc./ B. Tech./ B. Sc.
Experience: Fresher
Employment Type: Short Term / Long Term
Location: Gurugram, Haryana

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